Carbon Wikipedia

Carbon (from Latin: carbo "coal") is a chemical element with the symbol C and atomic number 6. It is nonmetallic and tetravalent —making four electrons available to form covalent chemical bonds . It belongs to group 14 of the periodic table. Three isotopes occur naturally, 12 C and 13 C being stable, while 14 C is a radionuclide , decaying with a halflife of about 5,730 years. Carbon is one ...

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Materials science Wikipedia

Materials science is a highly active area of research. Together with materials science departments, physics, chemistry, and many engineering departments are involved in materials research. Materials research covers a broad range of topics – the following nonexhaustive list …

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Silicon carbide fibermediated stable transformation of ...

Maize (Zea mays, cv ‘Black Mexican Sweet’) (BMS) and tobacco (Nicotiana tabacum, cv ‘Xanthi’) tissue cultures were transformed using silicon carbide fibers to deliver DNA into suspension culture cells. DNA delivery was mediated by vortexing cells in the presence of silicon carbide fibers and plasmid DNA.

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Robust crop resistance to broadleaf and grass herbicides ...

Nov 23, 2010· Silicon carbide fibermediated transformation of maize. DNA fragment containing the genetically engineered aad1 gene cassette was released from plasmid pDAS1740 by FseI digestion and introduced into recipient maize line HiII through silicon carbide fiber (Whiskers)mediated transformation . Immature embryos of maize were aseptically removed ...

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Plant transformation methods SlideShare

Jun 03, 2013· Silicon carbide fibre mediated micro m dia, 10 – 80 micro m lth. vortexing in an eppendorf tube a mixture of plasmid DNA encodinga selectable or screen able marker gene, si fibre and the explantstissue. DNA delivery in his system is presumably due to cell wall penetrationby DNA coated si C F. cellular penetration is likely enhanced by ...

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Transformation of plant cells Zeneca Limited

Nov 07, 1995· Asano et al, "Electroporationmediated and silicon carbide fibermediated DNA delivery in Agrostis alba L. (Redtop)", Plant Science, vol. 79, pp. 247252, 1991. Kaeppler et al, "Silicon carbide fibermediated stable transformation of plant cells…

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MOLECULAR BIOLOGY BIOTECHNOLOGY GENE TRANSFER …

polyethylene glycol method, microinjection, silicon carbide mediated transformation, liposome mediated gene transfer and ... and silicon carbide fibremediated transformation.

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Subculture vs Counterculture What's the difference ...

Verb (subcultur) (biology) To transfer (microorganisms) to a fresh growth medium in order to start a new culture * {{quotebook, 1990, Heidi F. Kaeppler et al., Silicon carbide fibermediated DNA delivery into plant cells, Plant Cell Reports citation, passage=Cultures were subcultured by 20fold dilution into fresh MS2D medium approximately every 7 d.

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Silicon carbide Wikipedia

Silicon carbide is an important material in TRISOcoated fuel particles, the type of nuclear fuel found in high temperature gas cooled reactors such as the Pebble Bed Reactor. A layer of silicon carbide gives coated fuel particles structural support and is the main diffusion barrier to the release of fission products.

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silicon carbide fibre mediated transformation wikipedia

Read "Transgenic plants of Lolium multiflorum , Lolium perenne , Festuca arundinacea and Agrostis stolonifera by silicon carbide fibremediated transformation of cell ... Chat Here. Transformation of Maize Using Silicon Carbide Whiskers ... Transformation of Maize Using Silicon Carbide ... K., Tetsuo, M, and Masaki, I. (1996) Silicon carbide ...

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(PDF) Silicon Carbide Whiskermediated Plant Transformation

for silicon carbide whisker mediated plant transformation. Asano et al. (1991) used the fiber technique to transform a suspension culture of Agrostis alba L. (Redtop).

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Silicon Wikipedia

Silicon carbide is rapidly attacked by chlorine gas, which forms SiCl 4 and carbon at 100 °C and SiCl 4 and CCl 4 at 1000 °C. It is mostly used as an abrasive and a refractory material, as it is chemically stable and very strong, and it fractures to form a very sharp cutting edge.

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(PDF) Gene Transfer Technologies in plants: Roles in ...

physical procedures of DNA introduction, including Recombinant DNA technologies circumvent these electroporation of protoplasts and tissues, microinjection limitations by enabling plant geneticts to identify and and silicon carbide fibremediated transformation. clone specific genes for desirable traits.

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Silicon carbide fibermediated stable transformation of ...

Silicon carbide fibermediated stable transformation of plant cells * H. E Kaeppler 1, Somers 1, Rines 2, and A. E Cockburn 3 Department of Agronomy and Plant Genetics and Plant Molecular Genetics Institute, University of Minnesota, 411 Borlaug Hall,

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Use of silicon carbide fibers for Agrobacteriummediated ...

Silicon carbide fibers have been used for causing wounds in the immature wheat embryos for Agrobacteriummediated genetic transformation. Immature embryos were put in a 5% silicon carbide fiber (SCF) suspension and vortexed for 2 or 3 min followed by cocultivation with Agrobacterium tumefaciens strain LBA4404.

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Maize transformation utilizing silicon carbide whiskers: a ...

We review here the most recently developed technique for maize transformation which involves the vortexing of silicon carbide whiskers with maize cells in the presence of plasmid DNA. Fertile transgenic plants have been regenerated following whiskermediated transformation which is compared with the alternatives described to date, namely ...

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ALTERNATIVE METHODS OF PLANT TRANSFORMATION …

considered the serious damage to transformed tissue by silicon carbide fibers to be the main reason for the unsatisfactory results. The abovementioned reports clearly indicate a low efficiency of silicon carbide fibermediated transformation as the main limitation for its practice. However, SCMT is an easy, fast and inexpensive procedure.

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Transgenic plants of Lolium multiflorum, Lolium perenne ...

The various stages in the silicon carbide fibremediated transformation of cell suspension cultures of Lolium multiflorum are shown in Fig. 1(a–j). Analogous procedures were used to recover transgenic plants of L. perenne, Festuca arundinacea and Agrostis stolonifera (Fig. 1 (k–m)).

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Pollenmediated method for transformation of maize, tomato ...

Oct 19, 2004· Silicon Carbide FiberMediated Genetic Transformation in Maize. Maize transformation via the pollination pathway was initiated in order to improve the pollinationbased transformation technique. Several plasmids with different selectable markers were used in pollination experiments of 600 maize plants of three different lines.

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Ceramic engineering Wikipedia

Ceramic engineering is the science and technology of creating objects from inorganic, nonmetallic materials. This is done either by the action of heat, or at lower temperatures using precipitation reactions from highpurity chemical solutions.

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Silicon Carbide WhiskerMediated Transformation of Cotton ...

Request PDF on ResearchGate | Silicon Carbide WhiskerMediated Transformation of Cotton (Gossypium hirsutum L.) | Plant transformation methods are invaluable biotechnological tools to …

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Silicon carbide fibermediated DNA delivery into plant ...

Silicon carbide fibermediated delivery of DNA into intact plant cells was investigated. Black Mexican Sweet (BMS) maize (Zea mays) and tobacco (Nicotiana tabacum) suspension culture cells were vortexed in the presence of liquid medium, plasmid DNA encoding βglucuronidase (GUS), and silicon carbide fibers. Penetration of BMS cells by the ...

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Boron Wikipedia

Boron is a useful dopant for such semiconductors as silicon, germanium, and silicon carbide. Having one fewer valence electron than the host atom, it donates a hole resulting in ptype conductivity. Traditional method of introducing boron into semiconductors is via its …

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Physical Methods of Gene Transfer | Genetics

ADVERTISEMENTS: The following points highlight the ten main physical methods of gene transfer. The methods are: 1. Biolystic or Particle Bombardment 2. Electroporation 3. Microinjection 4. Pollen Transformation 5. Liposome Mediated Transfer 6. Microlaser 7. MacroInjection 8. Silicon Carbide Fiber (SCF) Mediated Transfer 9. Poly Ethylene Glycol (PEG) Mediated Transformation 10.

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Electroporationmediated and silicon carbide fiber ...

Plant Science. 79 (1991) 247252 Elsevier Scientific Publishers Ireland Ltd. 247 Electroporationmediated and silicon carbide fibermediated DNA delivery in Agrostis alba L. (Redtop) Yoshito Asanoa, Yoshiaki Otsukib and Masashi Ugaki` "Department of Cell …

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Reinforced carbon–carbon Wikipedia

Carbon fibrereinforced silicon carbide (C/SiC) is a development of pure carbon–carbon, and can be used in automotive applications, such as components of brake systems on high performance road cars, namely the brake disc and brake pads. C/SiC utilises silicon carbide with carbon fibre, and this compound is thought to be more durable than pure carboncarbon.

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Silicon carbide fibers Wikipedia

Silicon carbide fibers fibers range from 5 –150 micrometres in diameter and composed primarily of silicon carbide molecules. Depending on manufacturing process, they may have some excess silicon or carbon, or have a small amount of oxygen. Relative to organic fibers and some ceramic fibers, silicon carbide fibers have high stiffness, high tensile strength, low weight, high chemical ...

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Subculture vs Fandom What's the difference? | WikiDiff

Verb (subcultur) (biology) To transfer (microorganisms) to a fresh growth medium in order to start a new culture * {{quotebook, 1990, Heidi F. Kaeppler et al., Silicon carbide fibermediated DNA delivery into plant cells, Plant Cell Reports citation, passage=Cultures were subcultured by 20fold dilution into fresh MS2D medium approximately every 7 d.

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(PDF) Silicon carbide fibermediated stable transformation ...

Silicon carbide fibermediated stable transformation of plant cells Article (PDF Available) in Theoretical and Applied Genetics 84(5):560566 · August 1992 with 332 Reads How we measure 'reads'

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Silicon carbide fibermediated DNA delivery into plant cells

Silicon carbide fibermediated delivery of DNA into intact plant cells was investigated. Black Mexican Sweet (BMS) maize (Zea mays) and tobacco (Nicotiana tabacum) suspension culture cells were vortexed in the presence of liquid medium, plasmid DNA encoding 13glucuronidase (GUS), and silicon carbide …

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Silicon carbide fibermediated DNA delivery into cells of ...

Silicon carbide fibermediated DNA delivery into cells of wheat (Triticum acstivum L.) mature embryos. ... using silicon carbide fibers (SCF). ... The potential of this new approach for stable transformation of wheat is under investigation. It has been found that callus tissues induced from the SCF treated embryos contain GUSexpressing sectors ...

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Subculture vs Microculture What's the difference? | WikiDiff

Verb (subcultur) (biology) To transfer (microorganisms) to a fresh growth medium in order to start a new culture * {{quotebook, 1990, Heidi F. Kaeppler et al., Silicon carbide fibermediated DNA delivery into plant cells, Plant Cell Reports citation, passage=Cultures were subcultured by 20fold dilution into fresh MS2D medium approximately every 7 d.

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